Flow Cytometry. First Principles

Flow cytometry: principles and instrumentation.

The standard benchtop flow cytometer is very similar to a hematology cell counter. In fact, flow cytometers can trace their origins back to the early hematology counters. Unlike their earlier counterparts that used electronic impedance to measure particles in a fluid stream, today’s modern flow cytometers use an illuminating light source usually consisting of a laser or arc lamp. The majority o...

Flow cytometry: basic principles and applications.

Flow cytometry is a sophisticated instrument measuring multiple physical characteristics of a single cell such as size and granularity simultaneously as the cell flows in suspension through a measuring device. Its working depends on the light scattering features of the cells under investigation, which may be derived from dyes or monoclonal antibodies targeting either extracellular molecules loc...

Technical Background and Methodological Principles of Flow Cytometry

Flow cytometry: principles and clinical applications in hematology.

The use of flow cytometry in the clinical laboratory has grown substantially in the past decade. This is attributable in part to the development of smaller, user-friendly, less-expensive instruments and a continuous increase in the number of clinical applications. Flow cytometry measures multiple characteristics of individual particles flowing in single file in a stream of fluid. Light scatteri...

Flow Cytometry

Flow cytometry is a technology that has impacted both basic cell biology and clinical medicine in a very significant manner. The essential principle of flow cytometry is that single particles suspended within a stream of liquid are interrogated individually in a very short time as they pass through a light source focused at a very small region. The optical signals generated are mostly spectral ...